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This blood test measures unconjugated estriol (E3) levels in serum. It is mainly used in prenatal screening to assess fetal well-being and the risk of chromosomal abnormalities. Estriol is produced by the placenta and fetus, so abnormal levels may indicate placental or fetal health issues. The test is commonly included in triple or quad marker screening during pregnancy. Results help doctors evaluate pregnancy progression and fetal risk.