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The Fasting Insulin test is a quantitative immunoassay performed on serum to measure insulin levels after an overnight fast. It is used to evaluate pancreatic beta-cell function and to assess insulin resistance, prediabetes, and diabetes mellitus. This test also helps in the investigation of metabolic syndrome, polycystic ovarian syndrome (PCOS), and hypoglycemic disorders. Results should be interpreted in conjunction with fasting glucose levels and clinical findings.